Research interests in our laboratory are in biofilm growth and control in drinking and industrial water systems; the fate, transport and survival of pathogens in biofilm systems; and the physiology of biofilm bacteria. Our focus has been primarily in low-nutrient aquatic environments. We have been using and developing methods for use in environmental systems that include RT-PCR, microarrays, community profile analysis by DGGE, distinguishing live/dead cells by PCR analysis. For determining physiological aspects of biofilm vs. suspended cells, methods development has included microarrays and proteomics, with model organisms being Staphylococcus aureus, Pseudomonas aeruginosa, and Salmonella typhimurium. We also have a focus in integrating information obtained on biofilm formation using the scanning confocal laser microscope with models that can be used to predict biofilm behavior. Recent work also includes investigating the microbial ecology of nitrification in oligotrophic water systems using traditional culturing and molecular methods.
Nocker, A., K. Sossa and A.K. Camper. 2007. Molecular monitoring of disinfection efficacy. J. Molecular Methods. 70:252-260.
Nocker, A., P. Sossa, M. Burr and A.K. Camper. 2007. Use of propidium monoazide for live-dead distinction in microbial ecology. Appl. Environ. Microbiol 73:5111-5117.
Klayman, B.J, P.A. Volden, P.S. Stewart and A.K. Camper. 2009. Escherichia coli O157:H7 requires colonizing partner to adhere and persist in a capillary flow cell. Environ. Sci. Technol. 43(6): 2105–2111.
Nocker, A., A. Mazza, L. Masson, A.K. Camper and R. Brousseau. 2009. Selective detection of live bacteria combining propidium monoazide sample treatment with microarray detection. J. Microbiol. Methods 76:253-261
Leach, L.H., P. Zhang, T.M. LaPara, R.M. Hozalski and A.K. Camper. 2009. Detection and enumeration of haloacetic acid degrading bacteria in drinking water distribution systems using dehalogenase genes. Applied Microbiology. 107(3):978–988
Zhang, P., L. H. Leach, A.K. Camper, E.H. Goslan, S. A. Parson, Y.F. Xie, R.M. Hozalski and T.M. LaPara. 2009. Accepted. Isolation and characterization of novel haloacetic acid-degrading bacteria from drinking water. FEMS Microbiology Letters.